Journal article
Induction of multi-functional T cells in a phase I clinical trial of dendritic cell immunotherapy in hepatitis C virus infected individuals
S Li, S Roberts, M Plebanski, M Gouillou, T Spelman, P Latour, D Jackson, L Brown, RL Sparrow, HM Prince, D Hart, BE Loveland, EJ Gowans
Plos One | Published : 2012
Abstract
We have previously reported a world-first phase I clinical trial to treat HCV patients using monocyte-derived dendritic cells (Mo-DC) loaded with HCV-specific lipopeptides. While the brief treatment proved to be safe, it failed to reduce the viral load and induced only transient cell-mediated immune responses, measured by IFNγ ELIspot. Here we reanalysed the PBMC samples from this trial to further elucidate the immunological events associated with the Mo-DC therapy. We found that HCV-specific single- and multi-cytokine secreting T cells were induced by the Mo-DC immunotherapy in some patients, although at irregular intervals and not consistently directed to the same HCV antigen. Despite the ..
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Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
[ "The funding for this work was provided by grants from 1. National Health and Medical Research Council (NHMRC) of Australia, ID 433908; 2. National Institutes of Health RO1A1054459; and 3. Private donations. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.", "The authors thank Calum de Burgh (University of Aberdeen, UK) for general technical assistance; Laurie Lamoreaux (Vaccine Research Centre, NIH) for critical advice on data interpretation; and Geza Paukovics (Burnet Institute) for assistance in flow cytometry. The authors gratefully acknowledge the contribution to this work of the Victorian Operational Infrastructure Support Program received by the Burnet Institute. EJG is a recipient of a NHMRC Senior Research Fellow award. The following reagent was obtained through BEI Resources, NIAID, NIH: Peptide Arrays for Hepatitis C Virus H77." ]